Transient expression ofDrosophila melanogasterrDNA promoter into culturedDrosophilacells
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منابع مشابه
Transient expression of Drosophila melanogaster rDNA promoter into cultured Drosophila cells.
Recombinant plasmids that carry the bacterial CAT gene under the transcriptional control of the D. melanogaster rDNA promoter have been introduced by transfection into cultured Schneider II Drosophila cells and their template activity followed at the RNA and protein level. While no CAT enzyme activity is measurable 48 hrs after transfection, high levels of hybrid rRNA-CAT transcripts that origi...
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Current procedures for introduction of DNA into Drosophila embryos require the time-consuming microinjection of DNA solution into individual embryos (1, 2). A procedure has recently been used successfully in plants, yeast and cultured cells for the ballistic introduction of DNA via tungsten (W) particles (3-5) . Using a modification of this procedure for Drosophila embryos, we have obtained tra...
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Spermatogenesis is a highly regulated process that involves both mitotic and meiotic divisions, as well as cellular differentiation to yield mature spermatozoa from undifferentiated germinal stem cells. Although Gpat2 was originally annotated as encoding a glycerol-3-phosphate acyltransferase by sequence homology to Gpat1, GPAT2 is highly expressed in testis but not in lipogenic tissues and is ...
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In order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three teleost fish cell lines. Several viral enhancers were found to be constitutively ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.16.6417